A primate model of Huntington's disease: cross-species implantation of striatal precursor cells to the excitotoxically lesioned baboon caudate-putamen
Identifieur interne : 000309 ( France/Analysis ); précédent : 000308; suivant : 000310A primate model of Huntington's disease: cross-species implantation of striatal precursor cells to the excitotoxically lesioned baboon caudate-putamen
Auteurs : O. Isacson [Royaume-Uni] ; D. Riche [France] ; P. Hantraye [France] ; V. Sofroniew [Royaume-Uni] ; M. Maziere [France]Source :
- Experimental Brain Research [ 0014-4819 ] ; 1989-03-01.
Abstract
Summary: Ibotenic acid was injected unilaterally into the baboon caudate-putamen (CP) to achieve a neural degeneration model in the primate, with a neuropathology similar to Huntington's disease. Four to six weeks later injections of cell suspensions of striatal precursor cells, obtained by dissection of the fetal rat striatal region (13–15 days gestational age), were made into the excitotoxically lesioned CP of 3 baboons immunosuppressed by Cyclosporin A. Morphological analysis indicated that in one of the baboons, which had the largest lesion of the CP and the shortest survival time (6 weeks after implantation), there was a surviving striatal implant. The implanted neurons grew in high densities in cellular aggregates within the host gliotic CP. These neurons had a neuronal size phenotypical for rat striatum, i.e. on average about a 25% smaller neuronal cell diameter than a similar population in the baboon caudate-putamen. Glial-fibrillary-acid-protein immunoreactivity was present on large astrocytes within the striatal implant, with a distinct border towards the lesion-induced astrogliosis of the host. Neuronal markers for acetylcholinesterase and Leu-enkephalin were distributed in a typical patchy manner in the striatal implants along with fiber staining for tyrosine-hydroxylase-like immunoreactivity (TH) possibly derived from afferent host dopaminergic axons. Some of these fibers in the implants came from intrinsic TH-positive neuronal somata, probably of neocortical fetal origin and transiently expressing the enzyme. In conclusion, the results indicate that neuronal replacement can be achieved by crossspecies implantation of fetal striatal precursor cells to the previously neuron depleted primate CP under immunosuppression but that the survival and growth of such implants may be variable and subject to unfavourable trophic conditions.
Url:
DOI: 10.1007/BF00248544
Affiliations:
- France, Royaume-Uni
- Angleterre, Angleterre de l'Est, Île-de-France
- Cambridge, Gif-sur-Yvette, Orsay
- Université de Cambridge
Links toward previous steps (curation, corpus...)
- to stream Main, to step Corpus: 002182
- to stream Main, to step Curation: 001E73
- to stream Main, to step Exploration: 002849
- to stream France, to step Extraction: 000309
Links to Exploration step
ISTEX:74ED9826924BDCC9E21366E8D866A5C691F31446Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">A primate model of Huntington's disease: cross-species implantation of striatal precursor cells to the excitotoxically lesioned baboon caudate-putamen</title><author><name sortKey="Isacson, O" sort="Isacson, O" uniqKey="Isacson O" first="O." last="Isacson">O. Isacson</name></author><author><name sortKey="Riche, D" sort="Riche, D" uniqKey="Riche D" first="D." last="Riche">D. Riche</name></author><author><name sortKey="Hantraye, P" sort="Hantraye, P" uniqKey="Hantraye P" first="P." last="Hantraye">P. Hantraye</name></author><author><name sortKey="Sofroniew, V" sort="Sofroniew, V" uniqKey="Sofroniew V" first="V." last="Sofroniew">V. Sofroniew</name></author><author><name sortKey="Maziere, M" sort="Maziere, M" uniqKey="Maziere M" first="M." last="Maziere">M. Maziere</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:74ED9826924BDCC9E21366E8D866A5C691F31446</idno><date when="1989" year="1989">1989</date><idno type="doi">10.1007/BF00248544</idno><idno type="url">https://api.istex.fr/document/74ED9826924BDCC9E21366E8D866A5C691F31446/fulltext/pdf</idno><idno type="wicri:Area/Main/Corpus">002182</idno><idno type="wicri:Area/Main/Curation">001E73</idno><idno type="wicri:Area/Main/Exploration">002849</idno><idno type="wicri:Area/France/Extraction">000309</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">A primate model of Huntington's disease: cross-species implantation of striatal precursor cells to the excitotoxically lesioned baboon caudate-putamen</title><author><name sortKey="Isacson, O" sort="Isacson, O" uniqKey="Isacson O" first="O." last="Isacson">O. Isacson</name><affiliation wicri:level="4"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Anatomy, University of Cambridge, Downing Street, CB2 3DY, Cambridge</wicri:regionArea><orgName type="university">Université de Cambridge</orgName><placeName><settlement type="city">Cambridge</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Angleterre de l'Est</region></placeName></affiliation></author><author><name sortKey="Riche, D" sort="Riche, D" uniqKey="Riche D" first="D." last="Riche">D. Riche</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Equipe de Neuroanatomie Fonctionelle, C.N.R.S., F-91198, Gif-sur-Yvette</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Gif-sur-Yvette</settlement></placeName></affiliation></author><author><name sortKey="Hantraye, P" sort="Hantraye, P" uniqKey="Hantraye P" first="P." last="Hantraye">P. Hantraye</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Département de Biologie, Service Hospitalier Frédéric Joliot, C.E.A., Institut de Recherche Fondamentale, F-91406, Orsay</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Orsay</settlement></placeName></affiliation></author><author><name sortKey="Sofroniew, V" sort="Sofroniew, V" uniqKey="Sofroniew V" first="V." last="Sofroniew">V. Sofroniew</name><affiliation wicri:level="4"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Anatomy, University of Cambridge, Downing Street, CB2 3DY, Cambridge</wicri:regionArea><orgName type="university">Université de Cambridge</orgName><placeName><settlement type="city">Cambridge</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Angleterre de l'Est</region></placeName></affiliation></author><author><name sortKey="Maziere, M" sort="Maziere, M" uniqKey="Maziere M" first="M." last="Maziere">M. Maziere</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Département de Biologie, Service Hospitalier Frédéric Joliot, C.E.A., Institut de Recherche Fondamentale, F-91406, Orsay</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Orsay</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Experimental Brain Research</title><title level="j" type="abbrev">Exp Brain Res</title><idno type="ISSN">0014-4819</idno><idno type="eISSN">1432-1106</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><date type="published" when="1989-03-01">1989-03-01</date><biblScope unit="volume">75</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="213">213</biblScope><biblScope unit="page" to="220">220</biblScope></imprint><idno type="ISSN">0014-4819</idno></series><idno type="istex">74ED9826924BDCC9E21366E8D866A5C691F31446</idno><idno type="DOI">10.1007/BF00248544</idno><idno type="ArticleID">Art21</idno><idno type="ArticleID">BF00248544</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0014-4819</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: Ibotenic acid was injected unilaterally into the baboon caudate-putamen (CP) to achieve a neural degeneration model in the primate, with a neuropathology similar to Huntington's disease. Four to six weeks later injections of cell suspensions of striatal precursor cells, obtained by dissection of the fetal rat striatal region (13–15 days gestational age), were made into the excitotoxically lesioned CP of 3 baboons immunosuppressed by Cyclosporin A. Morphological analysis indicated that in one of the baboons, which had the largest lesion of the CP and the shortest survival time (6 weeks after implantation), there was a surviving striatal implant. The implanted neurons grew in high densities in cellular aggregates within the host gliotic CP. These neurons had a neuronal size phenotypical for rat striatum, i.e. on average about a 25% smaller neuronal cell diameter than a similar population in the baboon caudate-putamen. Glial-fibrillary-acid-protein immunoreactivity was present on large astrocytes within the striatal implant, with a distinct border towards the lesion-induced astrogliosis of the host. Neuronal markers for acetylcholinesterase and Leu-enkephalin were distributed in a typical patchy manner in the striatal implants along with fiber staining for tyrosine-hydroxylase-like immunoreactivity (TH) possibly derived from afferent host dopaminergic axons. Some of these fibers in the implants came from intrinsic TH-positive neuronal somata, probably of neocortical fetal origin and transiently expressing the enzyme. In conclusion, the results indicate that neuronal replacement can be achieved by crossspecies implantation of fetal striatal precursor cells to the previously neuron depleted primate CP under immunosuppression but that the survival and growth of such implants may be variable and subject to unfavourable trophic conditions.</div></front></TEI><affiliations><list><country><li>France</li><li>Royaume-Uni</li></country><region><li>Angleterre</li><li>Angleterre de l'Est</li><li>Île-de-France</li></region><settlement><li>Cambridge</li><li>Gif-sur-Yvette</li><li>Orsay</li></settlement><orgName><li>Université de Cambridge</li></orgName></list><tree><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Isacson, O" sort="Isacson, O" uniqKey="Isacson O" first="O." last="Isacson">O. Isacson</name></region><name sortKey="Sofroniew, V" sort="Sofroniew, V" uniqKey="Sofroniew V" first="V." last="Sofroniew">V. Sofroniew</name></country><country name="France"><region name="Île-de-France"><name sortKey="Riche, D" sort="Riche, D" uniqKey="Riche D" first="D." last="Riche">D. Riche</name></region><name sortKey="Hantraye, P" sort="Hantraye, P" uniqKey="Hantraye P" first="P." last="Hantraye">P. Hantraye</name><name sortKey="Maziere, M" sort="Maziere, M" uniqKey="Maziere M" first="M." last="Maziere">M. Maziere</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/ParkinsonV1/Data/France/Analysis
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000309 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000309 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= ParkinsonV1
|flux= France
|étape= Analysis
|type= RBID
|clé= ISTEX:74ED9826924BDCC9E21366E8D866A5C691F31446
|texte= A primate model of Huntington's disease: cross-species implantation of striatal precursor cells to the excitotoxically lesioned baboon caudate-putamen
}}
| This area was generated with Dilib version V0.6.23. |  |